Testing Paternity Using DNA
2 Person Legal Paternity Test
This is a Legal DNA Paternity Test, and uses the most advanced technology to determine whether a biological relationship exists between an alleged father and a child. Test are available to test blood or saliva DNA.
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It is now possible to prove conclusively, one way or the other, if a man is indeed the father of a child thanks to DNA paternity testing.
DNA paternity testing works by isolating the DNA of both parents, and then comparing it with the child’s DNA. The comparison is similar to that of blood testing, but individual DNA is being examined, as opposed to broad blood types.
Early paternity tests were conducted using the blood types of each parent and that of the child, but they were inconclusive. If the father was Type A and the mother Type B, the only way the child might not be his, was if it was Type O. That left a huge margin for error or interpretation. DNA paternity testing on the other hand, is almost 100% conclusive in its results. It cannot tell the difference between two brothers’ particular DNA, but it can definitely show from which family the DNA originated. As long as the two possible parents in question are from different families, DNA paternity testing will work conclusively.
To isolate the DNA, a saliva sample is taken, and the DNA extracted using a restriction enzyme that cuts the DNA into identical and easily manageable lengths. The cut DNA pieces are then sorted according to size by the use of a special gel. The DNA is placed at one end of a slab of gelatin and is then drawn through the gel by an electric current. The gel acts like a sieve, allowing small DNA fragments to move more rapidly than larger ones.
The result is that after the gel has separated the DNA according to size, a blot is made to trap the DNA in their positions, with small DNA fragments near one end of the blot and large ones near the other end. The blot is now treated with another piece of DNA called a probe that binds to the DNA on the blot. If the patterns are identical, the result is a conclusive match.
